RNA

Part:BBa_K4501001:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)


anti-myc shRNA (1741-1761) C/D-box


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A C/D-box sequence was incorporated before the terminator to allow the loading of the shRNA into the exosomes via L7Ae.


Source

Designed by ourselves using the VectorBuilder software to design shRNA (https://en.vectorbuilder.com/design) on human MYC gene (NM_002467.6).

References